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United States Department of Agriculture

Agricultural Research Service

Research Project: Integrated Aquatic Animal Health Strategies

Location: Aquatic Animal Health Research

Title: Global transcription analysis of vaccinated channel catfish following challenge with virulent Edwardsiella ictaluri

Authors
item Pridgeon, Yuping
item Yeh, Hung-Yueh
item Shoemaker, Craig
item Klesius, Phillip

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 31, 2012
Publication Date: April 3, 2012
Repository URL: http://handle.nal.usda.gov/10113/55456
Citation: Wei Pridgeon, Y., Yeh, H., Shoemaker, C.A., Klesius, P.H. 2012. Global transcription analysis of vaccinated channel catfish following challenge with virulent Edwardsiella ictaluri. Veterinary Immunology and Immunopathology. 146:53-61.

Interpretive Summary: To understand which genes are involved in innate or adaptive immunity, microarray analysis were performed to compare gene expression in vaccinated channel catfish after challenge with a virulent bacterium compared to unvaccinated unchallenged control fish. A total of 167 functionally known genes were found to be up-regulated, whereas 40 were down-regulated. Microarray analysis revealed that lysozyme c was up-regulated the most (70- fold) in vaccinated fish at 48h post challenge of virulent bacterium E. ictaluri whereas myotubularin related protein 1a and cytochrome P450 2J27 were down-regulated the most (8.1 fold). Differential regulation of eight randomly selected transcripts in vaccinated fish after challenge with virulent E. ictaluri was also validated by quantitative PCR. Our results suggest that these differentially regulated genes might play important roles in channel catfish immunity against infections by bacterium E. ictaluri.

Technical Abstract: To determine the identities of genes involved in either innate or adaptive immunity, microarray analysis of 65,182 UniGene transcripts were performed to compare gene expression in vaccinated channel catfish after challenge with a virulent Edwardsiella ictaluri compared to that in sham-vaccinated fish without challenge. With a filter of false-discovery rate less than 0.05 and fold change greater than 2, a total of 167 functionally known unique transcripts were found to be up-regulated, whereas 40 were down-regulated. The 167 up-regulated transcripts represent genes with putative functions in the following eight major categories: (1) Immunity (30%); (2) Metabolism and energy production (22%); (3) Transcription or translation (12%); (4) Protein degradation (11%); (5) Signal transduction (6%); (6) Traffic and transport (6%); (7) Cell structure or cell cycle (8%); and (8) Others (5%). The 40 down-regulated transcripts represent genes with putative functions in the following six major categories: (1) Metabolism (27.5%); (2) Immunity (17.5%); (3) Cell structure (17.5%); (4) Cell motility (10%); (5) Signal transduction (15%); and (6) Others (12.5%). Microarray analysis revealed that lysozyme c was up-regulated the most (70- fold) in vaccinated fish at 48h post challenge of virulent E. ictaluri whereas myotubularin related protein 1a and cytochrome P450 2J27 were down-regulated the most (8.1 fold). Differential regulation of eight randomly selected transcripts in vaccinated fish after challenge with virulent E. ictaluri was also validated by quantitative PCR. Our results suggest that these differentially regulated genes might play important roles in channel catfish immunity against E. ictaluri.

Last Modified: 4/21/2014
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