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United States Department of Agriculture

Agricultural Research Service

Research Project: Rice as a Source and Delivery System for Nutrients and Lipophilic Bioactive Compounds

Location: Food Processing and Sensory Quality Research

Title: Glyceollin-elicited soy protein consumption induces distinct transcriptional effects as compared to standard soy protein

Authors
item Wood, Charles -
item Boue, Stephen
item Collins-Burow, Bridgette -
item Rhodes, Lindsay -
item Register, Thomas -
item Cline, J Mark -
item Dewi, Fitriya -
item Burow, Matthew -

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 29, 2011
Publication Date: January 11, 2012
Citation: Wood, C.E., Boue, S.M., Collins-Burow, B.M., Rhodes, L.V., Register, T.C., Cline, J., Dewi, F.N., Burow, M.E. 2012. Glyceollin-elicited soy protein consumption induces distinct transcriptional effects as compared to standard soy protein. Journal of Agricultural and Food Chemistry. 60(1):81-6.

Interpretive Summary: Glyceollins are stress-induced compounds in soybeans with bioactive properties distinct from parent soy isoflavones. The goals of this study were to evaluate effects of dietary glyceollin-enriched and standard soy protein isolates and identify candidate target pathways of glyceollins on transcriptional profiles within mammary gland tissue. Thirty female postmenopausal monkeys were randomized to diets containing one of three protein sources for 3 weeks: (1) control; (2) standard soy protein containing isoflavones (SOY); and (3) glyceollin-enriched soy protein containing isoflavones + glyceollins (GLY). Relative to the control diet, the GLY diet resulted in greater numbers of differentially regulated genes which showed minimal overlap with those of SOY. Effects of GLY related primarily to pathways involved in lipid and carbohydrate metabolism. The GLY diet also resulted in lower serum total cholesterol. These preliminary findings suggest that glyceollin-enriched soy protein has divergent effects from standard soy.

Technical Abstract: Glyceollins are stress-induced compounds in soybeans with bioactive properties distinct from parent soy isoflavones. The goals of this study were to evaluate effects of dietary glyceollin-enriched and standard soy protein isolates and identify candidate target pathways of glyceollins on transcriptional profiles within mammary gland tissue. Thirty female postmenopausal cynomolgus monkeys were randomized to diets containing one of three protein sources for 3 weeks: (1) control casein / lactalbumin (C/L); (2) standard soy protein containing 194 mg/day isoflavones (SOY); and (3) glyceollin-enriched soy protein containing 189 mg/day isoflavones + 134 mg/day glyceollins (GLY). All diets contained a physiologic dose of estradiol (E2) (1 mg/day). All doses are expressed in human equivalents scaled by caloric intake. Relative to the control C/L diet, the GLY diet resulted in greater numbers of differentially regulated genes which showed minimal overlap with those of SOY. Effects of GLY related primarily to pathways involved in lipid and carbohydrate metabolism, including peroxisome proliferator-activated receptor (PPAR)-gamma and AMP-activated protein kinase (AMPK) signaling, adipocytokine expression, triglyceride synthesis, and lipase activity. Notable genes upregulated by the GLY diet included PPAR-gamma, adiponectin, leptin, lipin 1, and lipoprotein lipase. The GLY diet also resulted in lower serum total cholesterol, specifically non-high-density lipoprotein cholesterol, and increased serum triglycerides compared to the C/L diet. No effects of GLY or SOY were seen on serum insulin, adipocytokines, or vascular and bone turnover markers. These preliminary findings suggest that glyceollin-enriched soy protein has divergent effects from standard soy with some specificity for adipocyte activity and nutrient metabolism.

Last Modified: 12/20/2014