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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetics, Physiology, and Health Research to Improve Catfish Production

Location: Warmwater Aquaculture Research Unit

Title: Pre and postprandial changes in orexigenic and anorexigenic factors in channel catfish Ictalurus punctatus

Authors
item Peterson, Brian
item Waldbieser, Geoffrey
item Riley, Jr, Larry -
item Upton, Kelli -
item Kobayashi, Yasuhiro -
item Small, Brian -

Submitted to: General and Comparative Endocrinology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: February 20, 2012
Publication Date: March 20, 2012
Citation: Peterson, B.C., Waldbieser, G.C., Riley, Jr, L.G., Upton, K.R., Kobayashi, Y., Small, B.C. 2012. Pre and postprandial changes in orexigenic and anorexigenic factors in channel catfish Ictalurus punctatus. General and Comparative Endocrinology. 176(2):231-239.

Interpretive Summary: Ghrelin (GRLN), cocaine and amphetamine regulated transcript (CART), neuropeptide Y (NPY), and cholecystokinin (CCK) are peptides involved in the regulation of feeding in vertebrates. We examined changes in the expression of plasma GHRL and mRNAs encoding GRLN, CART, NPY, and CCK in channel catfish. Feeding increased abundance of brain CART mRNA out to 4 h with no effect observed in unfed fish. Brain NPY expression peaked at 0.5 h in both treatments. NPY expression then declined in fed fish but remained elevated in unfed fish. No differences in plasma or stomach GRLN expression were observed. Brain CCKa and CCKb expression increased after feeding. These results suggest CART, NPY, and CCK play roles in the regulation of channel catfish feeding.

Technical Abstract: Ghrelin (GRLN), cocaine and amphetamine regulated transcript (CART), neuropeptide Y (NPY), and cholecystokinin (CCK) are neuropeptides involved in the regulation of appetite and feeding in vertebrates. We examined pre- and postprandial changes in the expression of plasma GHRL and mRNAs encoding GRLN, CART, NPY, and CCK in channel catfish. Fish were entrained to eat at 0900 h for two weeks. Fish were then sampled at 0700, 0800, and 0900 h. Remaining fish were either offered feed at 0900 h (Fed) or fasted (Unfed). Fish sampling continued at 0.5, 1, 2, and 4 h post feeding. Feeding increased abundance of whole brain CART mRNA out to 4 h with no effect observed in unfed fish. Whole brain NPY expression peaked at 0.5 h in both treatments. NPY expression then declined in fed fish but remained elevated in unfed fish. No differences in plasma or stomach GRLN expression were observed. Two separate cDNAs for CCK were identified. Brain CCKa and CCKb expression increased after feeding. These results suggest CART, NPY, and CCK play roles in the regulation of channel catfish feeding. Taken together, these results provide new insights into the neural and gastroenteric mechanisms regulating appetite in channel catfish.

Last Modified: 7/12/2014