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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Melanin determination by high performance liquid chromatography (HPLC) for K. marxianus

Authors
item Hughes, Stephen
item Cox, Elby -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 5, 2011
Publication Date: December 5, 2011
Citation: Hughes, S.R., Cox, E. 2011. Melanin determination by high performance liquid chromatography (HPLC) for K. marxianus [abstract]. p. 2.

Technical Abstract: Ultraviolet light (UV) mutated K. marxianus was found to turn dark brown during a growth assay. This brown color was hypothesized to be melanin overproduction influenced by the UV exposure. Cell cultures were oxidized and HPLC analyzed to determine melanin concentrations. The resulting melanin concentration of dried cell culture reconstituted at 10% by mass are below the detection limit of 63.0ppm melanin when compared to the melanin standard obtained from octopus ink.

Last Modified: 12/17/2014
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