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United States Department of Agriculture

Agricultural Research Service

Research Project: Discovery and Development of Natural Product-based Weed Management Methods

Location: Natural Products Utilization Research

Title: Esps Gene Amplification Endows Resistance to Glyphosate in Italian Ryegrass (Lolium Perene Ssp Multiflorum) from Arkansas, Usa

Authors
item Salas, Reiofeli -
item Dayan, Franck
item Pan, Zhiqiang
item Watson, Susan
item Dickson, James -
item Scott, Robert -
item Burgos, Nilda -

Submitted to: Pest Management Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 19, 2012
Publication Date: July 19, 2012
Citation: Salas, R.A., Dayan, F.E., Pan, Z., Watson, S.B., Dickson, J.W., Scott, R.C., Burgos, N.R. 2012. ESPS gene amplification endows resistance to glyphosate in Italian ryegrass (Lolium perene ssp multiflorum) from Arkansas, USA. Pest Management Science. 68:1223-1230.

Interpretive Summary: Resistance to glyphosate in weed species is a major challenge for the sustainability of glyphosate use in crop and non-crop systems, and especially in glyphosate-resistant crops. A glyphosate-resistant Italian ryegrass population has been identified in Arkansas. We discovered that resistance was not due to a mutation in the EPSPS gene. Instead, the resistant plants are up to 25 more copies of EPSPS gene than the susceptible plants. This is the second plant species to evolve resistance to glyphosate by this mechanism.

Technical Abstract: Resistance to glyphosate in weed species is a major challenge for the sustainability of glyphosate use in crop and non-crop systems, and especially in glyphosate-resistant crops. A glyphosate-resistant Italian ryegrass population has been identified in Arkansas. This research was conducted to elucidate its resistance mechanism. We investigated resistant and susceptible plants from a population in Desha County, Arkansas (Des03).The amounts of glyphosate that reduced growth of resistant plants 50% (GR50) were 7 to 13 times greater than those of susceptible plants from the same population. The EPSPS gene did not contain any point mutation that has previously been associated with resistance to glyphosate, nor were there any other mutations on the EPSPS gene unique to the Des03 resistant plants. The resistant plants had 6-fold higher basal EPSPS enzyme activities than the susceptible plants, but their I50 values in response to glyphosate were similar. The resistant plants contained up to 25 more copies of EPSPS gene than the susceptible plants. The level of resistance to glyphosate correlated with increases in EPSPS enzyme activity and EPSPS copy number. Increased EPSPS enzyme activity and EPSPS gene amplification confer resistance to glyphosate in Des03 population. This is the first report of EPSPS gene amplification in glyphosate-resistant Italian ryegrass. Other resistance mechanism(s) may also be involved.

Last Modified: 9/23/2014