Technical Abstract: Plant micro(mi)RNAs and trans-acting small interfering (tasi)RNAs mediate posttranscriptional silencing of genes and play important roles in a variety of biological processes. Although bioinformatics prediction and small (s)RNA cloning are the key approaches used for identification of miRNAs, tasiRNAs and their targets, these resources are not readily available for application to researchers studying non-model plant species. We developed a publically available webserver for Solanaceae miRNA/tasiRNA Analysis Resources and Tools (SMART), comprised of four bioinformatic tools linked to Solanaceae sequence databases. The server is designed to identify miRNAs and tasiRNAs that potentially regulate Solanaceae genes of interest. The webserver tools detect sRNAs, precursor miRNA (MIR) and tasiRNA (TAS) genes, cleavage products of miRNA and tasiRNAs, and map sRNAs onto input genes. These tools in conjunction with their associated databases can be used individually or in combination to identify and characterize MIR and TAS genes that target one or many genes of interest in Solanaceae species.