Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Abstract Only
Publication Acceptance Date: November 3, 2011
Publication Date: January 6, 2012
Citation: Wagner, T.A., Liu, J., Stipanovic, R.D., Puckhaber, L.S., Bell, A.A. 2012. Hemigossypol is present in glanded cottonseed (Gossypium hirsutum) [Abstract]. Proceedings of the Beltwide Cotton Conferences, January 3-6, 2012, Orlando, Florida. p. 12645. Technical Abstract: Gossypol is a dimeric sesquiterpenoid first identified in cottonseed, but found in various tissues in the cotton plant including the seed. From its first discovery, it was assumed that hemigossypol was the biosynthetic precursor of gossypol. Previous studies established that peroxidase (either from horseradish or from cottonseed) converts hemigossypol to gossypol. However, hemigossypol has never been identified in healthy cottonseed. In a temporal study using HPLC and LCMS, hemigossypol was identified in the developing cotton embryo and was shown to coincidently accumulate with gossypol and with delta-cadinene synthase and 8-hydroxy-delta-cadinene synthase, genes involved in the biosynthesis of hemigossypol. Taken together with previous experiments, this establishes hemigossypol as the biosynthetic precursor of gossypol.