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United States Department of Agriculture

Agricultural Research Service

Research Project: VIRUS-VECTOR-HOST INTERACTIONS OF ARBOVIRAL DISEASES OF LIVESTOCK

Location: Arthropod-Borne Animal Diseases Research

Title: Whole genome sequence analysis of field strains of bluetongue virus

Authors
item Wilson, William
item Smith, Timothy

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 12, 2010
Publication Date: November 11, 2010
Citation: Wilson, W.C., Smith, T.P. 2010. Whole genome sequence analysis of field strains of bluetongue virus. Meeting Proceedings. 154.

Technical Abstract: The Arthropod Borne Animal Diseases Research Unit(ABADRU) has been developing rapid, high sensitive biosenor technology based on gold nanoparticles and Surface Enhanced Raman Scattering (SERS). The “proof-of-concept” for the nucleic acid and immunological based assays was reviewed. A more recent enhancement in this technology was also discusses. The ABADRU has also adapted the published single primer ligation-whole genome amplification protocol that allows the whole bluetongue virus genome to be amplified without prior sequence knowledge and submitted to high-throughput DNA sequencing. Preliminary data was discussed as well as the potential impact on the ability to rapidly perform molecular evolution analyses.

Last Modified: 9/22/2014