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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING ARTHROPODS: INTEGRATED PEST MANAGEMENT

Location: Mosquito and Fly Research Unit

Title: Impact of deltamethrin-impregnated container covers on Aedes aegypti oviposition

Authors
item Kline, Daniel
item Clark, Gary
item Urban, Joyce
item Golden, Frances
item Nathan, Michael -
item Knox, Tessa -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 3, 2010
Publication Date: N/A

Technical Abstract: USDA researchers are studying novel methods to control Aedes aegypti. One approach focuses on prevention of oviposition by female Ae. aegypti. In collaboration with Vestergaard Frandsen Ltd., deltamethrin-treated PermaNet® Container Covers (jar lids) were evaluated with different configurations of 55-gallon drums with and without covers. Exclusion efficacy was measured with sticky ovitraps and oviposition substrates placed on the inner wall of the drums. Tests were performed in 1,800 ft2 outdoor screened cages (30 ft wide x 60 ft long, height 16 ft gabled to 18 ft) at the USDA CMAVE facilities in Gainesville, Florida. In test 1, there was 1 drum per cage and it had an untreated cover, a deltamethrin-treated drum cover, or was uncovered drum (untreated control). In test 2, there were 4 drums per cage in the following configurations: 3 covered drums and 1 open drum, 1 covered drum and 3 uncovered drums, or 4 uncovered drums (untreated control). Test 3 was similar to test 2 but with a different Container Cover. For each test, 200 gravid Ae. aegypti were released into each cage. The drum(s) were 2/3 full of well water and lined with absorbent germination papers to detect female oviposition. Container Covers efficacy was measured 24 hrs after the females were released when 5 sticky oviposition traps containing (a 10%) 7 day-old hay infusion were widely distributed in each cage as alternative oviposition sites for gravid females. Egg (germination) papers were removed after 72 hrs and the sticky traps were examined after 48 hrs. Drums with no cover or untreated covers yielded similar numbers of females; treated covers produced a 64% reduction in females. With 1 of 4 drums with treated covers, there was a 45 or 65% reduction in females and a 42 or 52% reduction in oviposition. With 3 of 4 drums with treated covers there was a 67 or 100% reduction in females and a 75 or 100% reduction in oviposition. The presence of the treated Container Covers significantly reduced female oviposition. This tool may result in better vector control and prevention of dengue virus transmission.

Last Modified: 12/18/2014