Technical Abstract: Lytic bacteriophages, viruses which infect and lyse bacterial cells, can provide a natural method to reduce bacterial pathogens on produce commodities. The use of multi-phage cocktails is most likely to be effective against bacterial pathogens on produce commodities, and minimize the development of phage resistance in target pathogens. Regulatory approval for the use of several lytic phage products on foods and in food processing environments has been granted by various U.S. agencies, which may lead to the more widespread use of bacteriophages in the decontamination of fresh and minimally processed produce. Bacteriophages are present in large numbers in the environment and foods, and recent human clinical trials have shown that they pose a minimal health risk to humans. Bacteriophages specific for Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes have been effective in reducing pathogen populations on produce commodities.