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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVED PLANT GENETIC RESOURCES FOR PASTURES AND RANGELANDS IN THE TEMPERATE SEMIARID REGIONS OF THE WESTERN U.S.

Location: Forage and Range Research

Title: Usda, ARS Cucumis Hystrix-Derived U.S. Processing Cucumber Inbred Backcross Line Population

Authors
item Staub, Jack
item Delannay, Isabelle -

Submitted to: HortScience
Publication Type: Germplasm Registration
Publication Acceptance Date: June 17, 2011
Publication Date: October 10, 2011
Citation: Staub, J.E., Delannay, I.Y. 2011. USDA, ARS Cucumis hystrix-derived U.S. processing cucumber inbred backcross line population. HortScience. 46:1428-1430.

Interpretive Summary: A series of 94 Cucumis hystrix Chakr.-derived U.S. processing market type inbred backcross lines (IBL) were released in January 2011 by the Agricultural Research Service, United States Department of Agriculture. The IBL were developed by crossing a U.S. processing cucumber (C. sativus L.) and C. hystrix - derived (C. hystrix x C. sativus) line, and is made available to U.S. cucumber breeders to supply a source from which they may develop processing market types with increased genetic diversity and yield potential suitable for field production. These diverse IBL will be useful in genetic studies and/or to evaluate cross-progency derived from matings between C. hystrix (2n = 2x = 24) or its derived germplasm [2n = 4x = 38 (amphidiploid C. hytivus) and 2n = 2x = 14 (C. hytivus x C. sativus backcross derivatives)] and C. sativus (2n = 2x = 14) during the development of improved cucumber germplasm. The 94 inbred backcross lines (IBL) were developed by crossing U.S. Department of Agriculture, Agricultural Research Service line WI 7023A (Madison, Wisconsin; recurrent parent) and the C. hystix-derived line 7012A (donor parent), and then selecting the most genetically diverse BC1 (30 of 392 individuals) based on molecular marker profiles (16 mapped loci). These selected BC1 individuals were pollinated by WI 7023A to produce 25 BC2 progeny. Approximately eight seeds per BC2 line were randomly selected for self-pollination to produce the BC2S1 generation followed by single seed descent to generate 94 BC2S3 IBL. The relatively high yielding, multiple lateral branching, gynoecious, determine line WI 7023A produces warty, light-green fruit of commercially unacceptable shape and quality. It was created by mating line Gy-7 (synom. G421) and line H-19, which were originally obtained from the University of Wisconsin-Madison (Madison, WI), and the University of Arkansas (Fayetteville, AR), respectively. Line WI 7023A was created through selection and backcrossing [Gy-7 (recurrent parent, University of Wisconsin) and H19 (donor parent, University of Arkansas (Fayetteville); BC4S3)] to identify a small-statured genotype for once-over mechanical harvest operations. It originated from the same populations that were used to develop recombinant inbred lines for the mapping of quantitative trait loci in U.S. processing cucumber. The late flowering, indeterminate, monoecious line WI 7012A is a BC1S3 line derived from a cross between the amphidiploid C. hytivus and the C. sativus long-fruited Chinese cv. Beijingjietou (recurrent backcross parent) mating. C. hytivus originated through the chromosome doubling of an in fertile F1 (C. hystrix x C. sativus, 2n = 2x = 19) individual to produce a fertile amphidiploid as identified via somaclonal variation during in vitro embryo culture.

Technical Abstract: A series of 94 Cucumis hystrix Chakr.-derived U.S. processing market type inbred backcross lines (IBL) were released in January 2011 by the Agricultural Research Service, United States Department of Agriculture. The IBL were developed by crossing a U.S. processing cucumber (C. sativus L.) and C. hystrix - derived (C. hystrix x C. sativus) line, and is made available to U.S. cucumber breeders to supply a source from which they may develop processing market types with increased genetic diversity and yield potential suitable for field production. These diverse IBL will be useful in genetic studies and/or to evaluate cross-progeny derived from matings between C. hystrix (2n = 2x = 24) or its derived germplasm [2n = 4x = 38 (amphidiploid C. hytivus) and 2n = 2x = 14 (C. hytivus x C. sativus backcross derivatives)] and C. sativus (2n = 2x = 14) during the development of improved cucumber germplasm. The 94 inbred backcross lines (IBL) were developed by crossing U.S. Department of Agriculture, Agricultural Research Service line WI 7023A (Madison, Wisconsin; recurrent parent) and the C. hystix-derived line 7012A (donor parent), and then selecting the most genetically diverse BC1 (30 of 392 individuals) based on molecular marker profiles (16 mapped loci). These selected BC1 individuals were pollinated by WI 7023A to produce 25 BC2 progeny. Approximately eight seeds per BC2 line were randomly selected for self-pollination to produce the BC2S1 generation followed by single seed descent to generate 94 BC2S3 IBL. The relatively high yielding, multiple lateral branching, gynoecious, determinate line WI 7023A produces warty, light-green fruit of commercially unacceptable shape and quality. It was created by mating line Gy-7 (synom. G421) and line H-19, which were originally obtained from the University of Wisconsin-Madison (Madison, WI), and the University of Arkansas (Fayetteville, AR), respectively. Line WI 7023A was created through selection and backcrossing [Gy-7 (recurrent parent, University of Wisconsin) and Hl9 (donor parent, University of Arkansas (Fayetteville); BC4S3)} to identify a small-statured genotype for once-over mechanical harvest operations. It originated from the same populations that were used to develop recombinant inbred lines for the mapping of quantitative trait loci in U.S. processing cucumber. The late flowering, indeterminate, monoecious line WI 7012A is a BC1S3 line derived from a cross between the amphidiploid C. hytivus and the C. sativus long-fruited Chinese cv. Beijingjietou (recurrent backcross parent) mating. C. hytivus originated through the chromosome doublng of an in fertile F1 (C. hystrix x C. sativus, 2n = 2x = 19) individual to produce a fertile amphidiploid as identified via somaclonal variation during in vitro embryo culture.

Last Modified: 8/27/2014
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