GENETIC AND GENOMIC APPROACHES TO IMPROVE PEANUT AND CORN RESISTANCE TO DISEASE AND AFLATOXIN CONTAMINATION
Location: Crop Protection and Management Research
Title: Genetic Linkage Map will aid the Whole genome Sequence Assembly
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 27, 2011
Publication Date: June 24, 2011
Citation: Guo, B., Qin, H., Feng, S., Chen, C.Y., Culbreath, A., Zhang, X., Holbrook Jr, C.C., Ozias-Akins, P., Liang, X. 2011. Genetic Linkage Map will aid the Whole genome Sequence Assembly. American Phytopathological Society Abstracts. 2011 American Phytopathological Society International Plant Protection Congress Joing Meeting in Honolulu, HI on August 6-10, 2011.
The allotetraploid peanut genome assembly will be a valuable resource to researchers studying polyploidy species, in addition to peanut genome evolution and domestication other than facilitating QTL analysis and the tools for marker-assisted breeding. Therefore, a peanut linkage map will aid genome assembly, acting as an independent resource against which contig assembly can be validated. The objective of this study was to develop a comparative integrated map from two recombinant inbred line populations. A total of 4576 SSR markers from three sources: published SSR markers, newly developed SSR markers from ESTs and from BAC end-sequences were used for screening polymorphisms. Two CAP markers were also included to differentiate ahFAD2A alleles and ahFAD2B alleles. A total of 324 markers were anchored on this integrated map covering 1,352.1 cM with 21 linkage groups (LGs). Combining information from duplicated loci between LGs and comparing with published diploid maps, 7 homoeologous groups were defined and 17 LGs (A1 to A10, B1 to B4, B7, B8, and B9) were aligned to corresponding A-subgenome or B-subgenome of diploid progenitors. One reciprocal translocation was confirmed in the tetraploid cultivated peanut genome. Several chromosomal rearrangements were observed. This genetic linkage map and others could provide a framework for QTL analysis and a scaffold for integration of the physical map and genome sequence assembly.