ARS | Publication request: An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 16, 2011
Publication Date: August 1, 2011
Citation: Klesius, P.H., Wei Pridgeon, Y. 2011. An in vitro screening method to evaluate chemicals as potential chemotherapeutants to control Aeromonas hydrophila infection in channel catfish. IN: 17th Annual USFWS Aquaculture Drug Approval Coordination Workshop, August 1-4, 2011, Bozeman, Montana. General Presentations 1-6.

Technical Abstract: Using catfish gill cells G1B and four chemicals (hydrogen peroxide, sodium chloride, potassium permanganate, and D-mannose), the feasibility of using an in vitro screening method to identify potential effective chemotherapeutants was evaluated in this study. In vitro screening results revealed that, at concentration of 100 mg L-1, H2O2 was the only chemical tested that was able to completely abolish the attachment and invasion of A. hydrophila to catfish gill cells. In vivo virulence studies using live channel catfish through bath immersion confirmed that H2O2 was the only chemical tested that was able to significantly (P<0.001) reduce the mortality (from 90 or 100% to 0 or 20%) caused by A. hydrophila infections. The in vitro screening method using catfish gill cells G1B could be used to initially identify potential effective chemotherapeutants to control A. hydrophila.