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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS

Location: Renewable Product Technology Research Unit

Title: Complete genome sequence of Lactobacillus buchneri NRRL B-30929, a novel strain from a commercial ethanol plant

Authors
item Liu, Siqing
item Leathers, Timothy
item Copeland, Alex -
item Chertkov, Olga -
item Goodwin, Lynne -
item Mills, David -

Submitted to: Journal of Bacteriology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 10, 2011
Publication Date: May 27, 2011
Repository URL: http://hdl.handle.net/10113/49856
Citation: Liu, S., Leathers, T.D., Copeland, A., Chertkov, O., Goodwin, L., Mills, D.A. 2011. Complete genome sequence of Lactobacillus buchneri NRRL B-30929, a novel strain from a commercial ethanol plant. Journal of Bacteriology. 193(15):4019-4020. DOI: 10.1128/JB.05180-11.

Interpretive Summary: A novel lactic acid bacterium Lactobacillus buchneri NRRL B-30929 was isolated as a contaminant of a fuel ethanol production facility. This organism uses a mixture of five-carbon and six-carbon sugars commonly found in agricultural residues and other lignocellulosic biomass. In this paper, the whole genome sequence of this organism was completed. The finished genome sequence will enable researchers to better understand carbohydrate metabolism and the stress response systems in this ethanol tolerant microbe and develop new biocatalysts for biomass-based biorefinery platforms.

Technical Abstract: Lactobacillus buchneri strain NRRL B-30929 was a contaminant obtained from a commercial ethanol fermentation. This facultative anaerobe is unique in its rapid growth on xylose and simultaneous fermentation of xylose and glucose. The strain utilizes a broad range of carbohydrate substrates and possesses a high tolerance to ethanol and other stresses, making it an attractive candidate for bioconversion of biomass substrates to various bioproducts. The genome sequence of NRRL B-30929 will provide insight into the unique properties of this lactic acid bacterium.

Last Modified: 12/28/2014
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