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United States Department of Agriculture

Agricultural Research Service

Research Project: EVALUATION OF THE QUALITY AND SAFETY OF TRANSGENIC SOYBEANS

Location: Soybean Genomics and Improvement

Title: Two-dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines

Authors
item Xi, Chen -
item Macdonald, Margaret
item Khan, Farooq
item Garrett, Wesley
item Matthews, Benjamin
item Natarajan, Savithiry

Submitted to: Proteomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 28, 2011
Publication Date: October 1, 2011
Citation: Xi, C., Macdonald, M.H., Khan, F.H., Garrett, W.M., Matthews, B.F., Natarajan, S.S. 2011. Two-dimensional proteome reference maps for the soybean cyst nematode Heterodera glycines. Proteomics. 11(24):4742-4746.

Interpretive Summary: The soybean cyst nematode (SCN) is the major pest of soybean in the U.S. To improve soybean yields by increasing the level of plant resistance to targeted pests, it is important to understand the protein composition in the SCN. We established a protein reference map for SCN using a proteomic approach in which SCN proteins are extracted, separated and identified. We have summarized the identified proteins and their putative functions. The identification of proteins in SCN will be useful to scientists who wish to develop nematode resistant soybeans.

Technical Abstract: Two-dimensional electrophoresis (2-DE) reference maps of Heterodera glycines were constructed. After in-gel digestion with trypsin, 803 spots representing 426 proteins were subsequently identified by LC-MS/MS. Proteins with annotated function were further categorized by Gene Ontology. Results showed that proteins involved in metabolic, developmental and biological regulation processes were the most abundant.

Last Modified: 4/19/2014
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