Title: Enrichment of chromosome 17 specific molecular markers of Pima cotton substituted in Upland cotton lines Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: April 17, 2011
Publication Date: May 1, 2011
Citation: Aziz, A.N., Kommireddy, V.L., Jenkins, J.N., McCarty Jr, J.C., Stelly, D.M., Saha, S. 2011. Enrichment of chromosome 17 specific molecular markers of Pima cotton substituted in Upland cotton lines. Proceedings 1890 Association of Research Directors, Biennial Research Symposium, April 9-11, 2011, Atlanta, GA. CD ROM. Technical Abstract: Cotton is the primary source of non-synthetic textile fiber, as well as an important source of food, feed, fuel and other products. In the USA cotton is a major crop in 13 states and grown in 17 states on about 5 million hectares, more than all crops except maize, wheat or soybean, with a return of about 6.5 billion dollars annually for its fiber and seed by products contributing to US agricultural security and sustainable agriculture. Two cultivated tetraploid species are vital for American cotton industry, i.e., Upland cotton (Gossypium hirsutum) and Pima cotton (G. barbadense) that are prized for high yields and exceptional fiber fineness, respectively. The overall objective of this research was to develop molecular map for fiber and agronomic traits of chromosome 17-specific recombinant-inbred line (CS-RIL) populations involving segments of chromosomes 17 from Gossypium barbadense in G. hirsutum genetic background. The CS-RILs were developed from crosses of CS-B17 G. barbadense chromosome substitution lines with their common recurrent parent, inbred G. hirsutum TM-1. The 47 CS-RILs with commercial varieties, DP393 and PHY 370 WR, were grown in four environments at USDA/ARS, Mississippi State, MS. In each environment, we used a randomized complete block design with four replications. Four agronomic and five fiber traits were measured and analyzed separately for the CS-RIL population. The amplified fragment length polymorphism (AFLP) markers were used to comparatively analyze the parents, CS-B17 and Upland cotton (TM-1) line, and 3-79, the donor parent of the alien chromosome (double haploid line) following the manufacturer’s protocol of AFLP kit. Thirty-two IRD-800 and IRD-700 labeled AFLP markers were used to genotype CS17-RIL populations. AFLP analyses of 47 recombinant inbred lines of CS-B17 along with two commercial varieties, DP-397 and PHY-370 WR, revealed potential association of some AFLP markers with micronaire quality trait. The CS-RIL population showed significant genetic diversity for all agronomic and fiber traits being investigated. Sixty percent of CS-B17 RI lines had lower micronaire than PHY 370 WR. These AFLP markers will be useful in marker assisted selection programs for this trait.