ARS | Publication request: Differences in responses and protein profiles of soybean Near Isogenic Lines (NILs) to Phakopsora pachyrhizi inoculation

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2011
Publication Date: August 6, 2011
Citation: Ganiger, M., Walker, D.R., Chen, Z. 2011. Differences in responses and protein profiles of soybean Near Isogenic Lines (NILs) to Phakopsora pachyrhizi inoculation [abstract]. American Phytopathological Society, International Plant Protection Congress Joint Meeting, August 6-10, 2011, Honolulu, Hawaii. 2011 CD ROM.

Technical Abstract: Asian soybean rust, caused by Phakopsora pachyrhizi, was first discovered in continental U.S. in late 2004. This pathogen has the potential to cause severe yield losses as all U.S. commercial soybean varieties are susceptible. In this study, ten Near Isogenic Lines (NILs) of three different populations were evaluated for differences in resistance to infection by P. pachyrhizi (Louisiana isolate). These lines, which had previously been evaluated against Florida soybean rust isolate, were evaluated in both growth chambers using detached leaves and under greenhouse conditions. For each line, sixteen plants were evaluated at R1 stage through inoculation with 200 µl spore suspension (3 x 104 spores/mL) with 0.01% Tween-20 per leaf on the upper surface. For detached leaf assay, soybean leaves at R1 stage were inoculated in the same manner. Fifteen days after inoculation, plants in green house and detached leaves in growth chamber were evaluated for lesion appearance, pustule formation, and pustule eruption and density. There was a significant difference among NILs in response to P. pachyrhizi infection in growth chamber and greenhouse conditions. Some of these lines are currently being compared for protein profile differences with and without soybean rust inoculation to identify potential proteins involved in soybean resistance to rust infection.