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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #265656

Title: Raspberry latent virus a plant reovirus that is aphid transmitted in a replicative persistent manner

Author
item QUITO-AVILA, D - Oregon State University
item Martin, Robert

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/25/2011
Publication Date: 8/2/2011
Citation: Quito-Avila, D.F., Martin, R.R. 2011. Raspberry latent virus a plant reovirus that is aphid transmitted in a replicative persistent manner. Phytopathology. 101(6):S149.

Interpretive Summary:

Technical Abstract: Raspberry latent virus (RpLV), is a newly characterized reovirus found in commercial raspberry fields in the Pacific Northwest (PNW). Phylogenetic analyses showed that RpLV is related most closely to Rice ragged stunt virus (RRSV), the type member of the genus Oryzavirus. RRSV and all members of plant reoviridae are transmitted by species of leafhoppers in a replicative persistent manner. After several failed attempts to transmit RpLV using leafhoppers, Amphorophora agathonica, the common raspberry aphid in the PNW, was tested as a vector of RpLV. RpLV was detected in aphids after a 12h-acquisition period using quantitative RT-PCR. Using a standard curve generated for quantifying RpLV, it was shown that the virus titer in aphids continued to increase after the acquisition period even when aphids were maintained on healthy plants with successive transfers onto fresh healthy plants every two days. This suggests that the virus replicates in the vector. Serial transfers of virus-carrying aphids to healthy plants demonstrated that the virus has a 7-day latent period in the aphid before it can be transmitted. A low percentage of plants tested positive for RpLV, 60 days post-inoculation, using aphids that tested positive for the virus, suggesting that aphids are inefficient vectors of this virus. Further experiments showed that RpLV is not transmitted transovarially to the next generation. To our knowledge this is the first report of an aphid transmitted plant reovirus.