Technical Abstract: Huanglongbing (HLB) is a serious and devastating disease of citrus caused by Candidatus Liberibacter spp. and vectored by the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). The disease has the potential to greatly limit the production of citrus in Florida and other citrus growing regions worldwide. Current control of D. citri and HLB is inadequate, but the identification and incorporation of D. citri resistance traits from uncultivated Citrus spp. and Citrus relatives is seen as a potential disease management strategy. In this study, 87 Rutaceae genotypes, primarily in the orange subfamily Aurantioideae, were assessed in the field for resistance to natural South Florida populations of D. citri. The majority of genotypes surveyed hosted all three life stages of D. citri, however there were significant differences among the genotypes in the mean ranks for D. citri eggs (F = 3.13, df = 86, P < 0.0001), nymphs (F = 9.01, df = 86, P < 0.0001), and adults (F = 4.21, df = 86, P < 0.0001). The only sampled genotype that was completely avoided by all life stages of D. citri was Casimiroa edulis Llave et Lex, commonly known as white sapote, which was one of the few plants included in the study belonging to the Rutaceae subfamily Toddalioideae. Although not completely avoided, very low levels of D. citri were found on two surveyed genotypes of Poncirus trifoliata (L.) Raf, Simmon’s trifoliate and Little-Leaf. Poncirus trifoliata, the trifoliate orange, readily forms hybrids with Citrus spp., is commonly incorporated into rootstock varieties, and has been used in breeding advance scion material. The identification of partial resistance in P. trifoliata to D. citri may prove useful in future citrus breeding efforts aimed at reducing the incidence and spread of HLB.