Technical Abstract: Maize (Zea mays L.) is an important agricultural crop. Various insect pests such as those in the order of Lepidoptera frequently feed on young maize plants and pose a significant threat to plant development and survival. To deal with this problem, maize generates a wide variety of responses to attack by pests, from activation of wound-response pathways such as jasmonic acid (JA) biosynthesis to the release of volatile compounds. Several maize lines have been developed that show resistance to the larvae of the fall armyworm [Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)]. Mp708, an inbred line resistant to feeding by fall armyworm, was developed through traditional breeding methods, but the mechanisms of resistance are not completely understood. Mp708 has been shown to have a moderately high constitutive expression of JA and other octadecanoid compounds prior to infestation by fall armyworm. On the other hand Tx601, a genotype susceptible to feeding by fall armyworm, activates JA pathway only in response to feeding, suggesting that Mp708 is “primed” to respond swiftly to attack. Current research indicates that fall armyworm larvae show a lack of preference to feeding on Mp708, leading to the hypothesis that volatiles released by the plant may also play an important role in its resistance. Analysis of the volatiles released by the resistant and susceptible lines in the presence and absence of the fall armyworm was conducted using Solid Phase Microextraction (SPME) in conjunction with gas chromatography mass spectrometry. We have demonstrated the presence of (E)-ß-caryophyllene, a terpenoid compound associated with resistance, in Mp708. In addition, 4-day-old fall armyworm larvae show a marked preference for Tx601 whorl tissue over Mp708 tissue. Identifying specific volatiles correlated with resistance to fall armyworm could lead to the integration of these traits into commercial maize hybrids.