NEWCASTLE DISEASE EPIDEMIOLOGY, PATHOGENESIS, AND CONTROL
Location: Exotic and Emerging Avian Viral Diseases Research Unit
Title: The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2011
Publication Date: May 15, 2011
Citation: Edwards, I.C., Susta, L., Cardenas, S., Miller, P.J., Brown, C.C., Afonso, C.L. 2011. The effect of IL-2 expression by recombinant Newcastle disease virus on host immune response, viral replication and pathogenesis [abstract]. 1st International Avian Respiratory Disease Conference, May 15-18, 2011, Athens, Georgia. p. 34.
Interleukin 2 (IL-2) is a soluble cytokine that stimulates the cell-mediated immune response. Virus constructs, such as recombinant vaccinia virus, expressing chicken IL-2 have been shown to improve viral clearance by natural killer cells in mice. We have inserted the open-reading frame of the chicken IL-2 gene into a viscerotropic velogenic Newcastle disease virus backbone (recombinant ZJ1 strain) in order to determine how the expression of IL-2 at the site of infection during viral replication affects the local and systemic immune response, viral replication and viral pathogenesis. Results from embryo inoculation (mean death time), intra-cranial pathogenecity index, cell culture multistep growth curve and chicken pathogenesis experiments will be presented.