NEWCASTLE DISEASE EPIDEMIOLOGY, PATHOGENESIS, AND CONTROL
Location: Exotic and Emerging Avian Viral Diseases Research Unit
Title: Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples
Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: February 15, 2011
Publication Date: July 16, 2011
Citation: Ecco, R., Brown, C.C., Edwards, I.C., Cagle, C.A., Miller, P.J., Susta, L., Pantin Jackwood, M.J., Afonso, C.L. 2011. Analysis of transcriptional responses of chickens infected with different Newcastle disease virus isolates using paraffin embedded samples [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. p. 14.
The transcriptional response of several cytokines in the spleen of chicken naturally infected by Newcastle Disease velogenic viscerotropic viruses was compared to the responses of atypical velogenic, velogenic neurotropic, and mesogenic strains during the first five days after infection. The ribonucleic acid expression for interferon gamma and interleukin 6 was enhanced at day two in the highly virulent velogenic viscerotropic viruses and corresponded with the presence of the virus in tissues. However, in one atypical velogenic viscerotropic, velogenic neurotropic and, a mesogenic virus the cytokine responses to infection were delayed or reduced. A pronounced increase of ribonucleic acid expression levels of interleukin 6 and interferon gamma was detected simultaneously with infiltration of macrophages and/or lymphoid necrosis in the histopathological analysis of the spleen and cecal tonsils.