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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMIC AND FUNCTIONAL ANALYSIS OF THE MUCOSAL IMMUNE RESPONSE AND ITS ROLE IN PROTECTION AGAINST RESPIRATORY PATHOGENS IN POULTRY

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Title: Evaluation of cytokine gene expression after avian influenza virus infection in avian cell lines and primary cell cultures

Authors
item Shepherd, Eric
item Cagle, Caran
item Pantin-Jackwood, Mary
item Faulkner, Olivia
item Wasilenko, Jamie
item Smith, Diane

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: March 15, 2011
Publication Date: July 16, 2011
Citation: Shepherd, E.M., Cagle, C.A., Pantin Jackwood, M.J., Faulkner, O.B., Wasilenko, J.L., Smith, D.M. 2011. Evaluation of cytokine gene expression after avian influenza virus infection in avian cell lines and primary cell cultures [abstract]. American Association of Avian Pathologists Annual Meeting, July 16-19, 2011, St. Louis, Missouri. CD-ROM.

Technical Abstract: The innate immune responses elicited by avian influenza virus (AIV) infection has been studied by measuring cytokine gene expression by relative real time PCR (rRT-PCR) in vitro, using both cell lines and primary cell cultures. Continuous cell lines offer advantages over the use of primary cell cultures, but it’s not clear if the results are comparable when studying the innate immune responses to virus infection. In this study, minor differences in cytokine gene expression and in viral replication were found when comparing two chicken-origin cell lines, DF-1 (fibroblast) and HD-11 (macrophage), to two primary cell cultures, chicken embryo fibroblast (CEF) and peripheral blood mononuclear cells (PBMC) when examined at 2, 8, and 24 hours post infection with a low pathogenic avian influenza virus, TK/WI/68 (H5N9).

Last Modified: 7/23/2014
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