ARS | Publication request: Virus-specific antibodies interfere with avian influenza infection in peripheral blood mononuclear leukocytes from young or aged chickens

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: July 1, 2011
Publication Date: July 17, 2011
Citation: Faulkner, O.B., Kapczynski, D.R., Pantin Jackwood, M.J., Cagle, C.A., Suarez, D.L. 2011. Virus-specific antibodies interfere with avian influenza infection in peripheral blood mononuclear leukocytes from young or aged chickens [abstract]. American Association of Avian Pathologists Meeting, July 16-19, 2011, St. Louis, Missouri. CD-ROM.

Technical Abstract: Avian influenza virus (AIV) infection was examined in peripheral blood mononuclear leukocyte cultures (PBMC) that were collected from 1-day-old chicks or from 52-week-old chickens. Virus-specific antibodies were incubated with AIV to model maternal antibody interference in vitro. Interferon-alpha (IFN-alpha), interleukin-1beta (IL-1beta), toll-like receptor (TLR) 7, and major histocompatibility complex class 1 (MHC1) genes were measured 24 hours after A/Turkey/Wisconsin/1968 (H5N9) low pathogenic AIV infection. Avian influenza increased all genes in PBMC from 52-week-old chickens; however TLR7 and MHC1 genes were lower in AIV infected PBMC from 1-day-old chicks. Virus-specific antibodies interfered with IFN-alpha more than IL-1beta in AIV infected PBMC.