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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING AND STINGING PESTS: ECOLOGY AND BIOLOGICALLY-BASED CONTROL

Location: Biological Control of Pests Research Unit

Title: A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination

Authors
item Liu, Y -
item Chen, Jian
item Guo, Y -
item Wang, C -
item Liang, X -
item Zhu, G -

Submitted to: Journal of Environmental Science and Health
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 9, 2010
Publication Date: May 1, 2011
Citation: Liu, Y.H., Chen, J., Guo, Y.R., Wang, C.M., Liang, X., Zhu, G.N. 2011. A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination. Journal of Environmental Science and Health. 46:313-320.

Interpretive Summary: Pesticide residual is a major source of food contamination. Routine monitoring of pesticide residue is needed to ensure the food safety. Due to their simplicity and cost-effectiveness, Immunoassays, especially the enzyme-linked immunosorbent assay (ELISA), have been emerging as an attractive alternative to the traditional chromatographic methods in pesticide residual analysis. A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. To the best of our knowledge, this assay has the best specificity among all the published researches on ELISAs for chlorpyrifos.

Technical Abstract: A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC50 and IC10 of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries recovery rates in seven agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8%. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published researches on ELISAs for chlorpyrifos.

Last Modified: 4/16/2014