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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGIES FOR PRODUCING RENEWABLE CHEMICALS AND POLYMERS FROM CARBOHYDRATES DERIVED FROM AGRICULTURAL FEEDSTOCKS

Location: Renewable Product Technology Research Unit

Title: Effect on Intestinal Growth of the Population of Cellobiose-Oligosaccharides Obtained by Enzymatic Reaction with Dextransucrase

Authors
item Ruiz-Matute, A -
item Brokl, M -
item Soria, A -
item Cote, Gregory
item Rastall, R -
item Sanz, M -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: December 17, 2010
Publication Date: December 17, 2010
Citation: Ruiz-Matute, A.I., Brokl, M., Soria, A.C., Cote, G.L., Rastall, R.A., Sanz, M.L. 2010. Effect on intestinal growth of the population of cellobiose-oligosaccharides obtained by enzymatic reaction with dextransucrase [abstract]. II Workshop Probioticos, Prebioticos y Salud. Evidencia Cientifica. Abstract #1.

Technical Abstract: Although the synthesis of oligosaccharides obtained by reactions catalyzed by dextransucrase using sucrose as donor and different carbohydrates as acceptors has been widely studied, the effect of many of these carbohydrates in the growth of intestinal flora has not yet been evaluated. Such is the case of gluco-oligosaccharides from the reaction between sucrose and cellobiose (non-digestible disaccharide), which have antimicrobial properties against dental caries and prevent the adherence of bacteria to teeth. In this work we have first studied the effect of oligosaccharides obtained by the action of Leuconostoc mesenteroides dextransucrase B-512F on cellobiose with respect to the growth of bacteria from fecal samples. Carbohydrates obtained were fractionated by size exclusion chromatography and characterized by GC-MS. These carbohydrates were subjected to small-scale in vitro tests using human feces from three healthy donors with a controlled diet. Bacterial growth was studied by fluorescence hybridization in vitro and production of fatty acids was assessed by HPLC. In general, the oligosaccharides studied had bifidogenic properties while no difference was observed in populations of Clostridia, Bacteroides, Lactobacillus, and Eubacteria. Nor were there significant differences in bacterial growth as a function of molecular weight of these compounds.

Last Modified: 10/24/2014
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