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United States Department of Agriculture

Agricultural Research Service

Research Project: CHARACTERIZATION OF HOST-PATHOGEN INTERACTIONS IN BARLEY AND WHEAT

Location: Cereal Crops Research

Title: Polyethylene glycol (PEG)-mediated transformation in filamentous fungal pathogens

Authors
item Zhaohui, Liu -
item Friesen, Timothy

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 22, 2011
Publication Date: N/A

Technical Abstract: Genetic transformation is an essential tool in molecular biology for many purposes including the study of gene function and the genetic improvement of an organism. The genetic transformation of many fungal species is a well established process that can be carried out by utilizing different transformation methods including electroporation, agrobacterium, biolistics, or polyethylene glycol (PEG) mediated transformation. Due to its simplicity in technical operation and equipment needed, PEG mediated transformation is still the most commonly used method for genetic transformation in filamentous fungi. Here, we describe a PEG-based protocol specifically developed for genetic transformation of Stagonospora nodorum, a fungal pathogen of wheat. This protocol can also be applied directly or with modification to other fungi especially those in the Dothideomycete class of fungi.

Last Modified: 9/21/2014
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