Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2010
Publication Date: December 16, 2010
Citation: Havey, M.J., Kielkowska, A.Z. 2010. Studies on in vitro culture of cucumber microspores [abstract]. Plant and Animal Genome Conference. Paper No. P871. Technical Abstract: Cucumber (Cucumis sativus L.) microspores are unique because they possess relatively few, large mitochondria and these mitochondria are paternally transmitted to progenies. We are working to develop microspore embryogenesis of cucumber microspores as possible targets for mitochondrial transformation. Microspores at the uninucleate stage were isolated from greenhouse-grown plants. A factorial design was performed including three accessions of cucumber, eight liquid culture media [MS (Murashige and Skoog), B5 (Gambrog) and NLN (Lichter) based] supplemented with various plant growth regulators, four culture densities (40-60-80-100 thousand microspores per ml), and stress treatments (cold and heat stress). Protocols for successful isolation and viability testing of cultured microspores were identified. Different conditions were tested for surface sterilization of male-flower buds, but it was not possible to obtain sterile cultures. However sterile plants were produced from in vitro from seed. The best growth conditions (medium, light, temperature) were identified to induce flowering of in vitro grown cucumber. Cytological observations of meiosis and pollen from in vitro produced flowers were performed. Although sterile cultures of uninucleate microspores were produced, no in vitro development of microspores has been observed.