|Duangjit, Janejira -|
|Bohanec, Borut -|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2010
Publication Date: December 16, 2010
Citation: Duangjit, J., Bohanec, B., Havey, M.J. 2010. Development of a haploid mapping family for onion [abstract]. Plant and Animal Genome Conference. Paper No. P668. Technical Abstract: Onion has one of the largest nuclear genomes among all diploid plants, approximately 63 times greater than rice. Pilot sequencing of the onion transcriptome revealed adequate numbers of single nucleotide polymorphisms (SNPs) to develop a high-density genetic map. We are presently sequencing normalized cDNAs from two parental lines of onion: 5225 is a red, doubled-haploid onion and was kindly provided by Seminis Seed Company and OH-1 is a yellow inbred that shows high frequencies of gynogenic haploid production. SNPs will be identified between these two parental cDNA populations and mapped using a new haploid mapping family. Individual plants of OH-1 and 5225 were crossed and seed was harvested from the OH-1 parent. From one cross, red bulbs were selected as hybrids and sent to the University of Ljubljana (Slovenia) for gynogenic haploid extraction. Highly pure DNA has been isolated from over 150 haploids for mapping. These haploids have also been asexually propagated and will be used for genetic analyses of bulb-quality traits.