INTERVENTIONS AND METHODOLOGIES TO REDUCE HUMAN FOOD-BORNE BACTERIAL PATHOGENS IN CHICKENS
Location: Poultry Microbiological Safety Research
Title: Salmonella Recovery Following Immersion Chilling for Matched Neck Skin and Whole Carcass Enrichment Sampling Methodologies
Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: October 15, 2010
Publication Date: January 24, 2011
Citation: Holmes, J.M., Buhr, R.J., Cason Jr, J.A., Cox Jr, N.A., Bourassa, D.V., Rigsby, L.L., Cray, P.J. 2011. Salmonella Recovery Following Immersion Chilling for Matched Neck Skin and Whole Carcass Enrichment Sampling Methodologies. International Poultry Scientific Forum. p.16.
The prevalence and serogroups of Salmonella recovered following immersion chilling were determined for both neck skin and the matching whole carcass enriched samples. Commercially processed and eviscerated broiler carcasses were immersion chilled in ice and tap water for 40 min. Following immersion chilling, each carcass was hung by the wing for 5 min to allow water to drip. From each carcass, the neck skin (8.3 g) was removed and stomached in 83 mL 1% buffered peptone water. The remaining carcass was subjected to whole carcass enrichment in 400 mL 1% buffered peptone water. Both the neck skins and whole carcasses were incubated at 37C for 24 h before aliquots were transferred to selective enrichment broths (RV and TT). Following incubation, BGS and MLIA plates were streaked and incubated at 37C for 24 h. From each plate, three colonies displaying typical Salmonella characteristics were individually stabbed into TSI and LIA slants. For neck skin samples, 12/40 were Salmonella-positive with 11 identified as serogroup C3 and 1 identified as serogroup B. For whole carcass enrichment, 37/40 were Salmonella-positive with 11 identified as serogroup B, 33 identified as serogroup C3, and 11 identified as serogroup D. Three different Salmonella serogroups were detected on two of the whole carcasses (C3/D/B) while no Salmonella was detected on the matching neck skin samples. Two different Salmonella serogroups were detected on twelve whole carcass samples either (C3/B) or (C3/D) while no Salmonella was detected on the corresponding neck skin samples. A representative group of D, B and C3 positive serogroups were serotyped and found to be S. Typhimurium, S. Kiambu, and S. Kentucky respectively. Of the three Salmonella-negative carcasses, only one corresponding neck skin was Salmonella-positive. For the 28 negative neck skin samples, 26 had Salmonella-positive matching whole carcasses. In this study, the whole carcass enrichment was superior to neck skin excision, detecting Salmonella on 92% versus 30% of the samples, respectively.