Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: October 3, 2010
Publication Date: February 1, 2011
Citation: Kindiger, B.K. 2011. Identifying the genetic relatedness of poa utilizing PAL-PCR[abstract]. Plant and Animal Genome Conference, January 15-19, 2011, San Diego, California. Paper No. P210. Available: http://www.intl-pag.org/19/abstracts/. Interpretive Summary: Abstract only.
Technical Abstract: Plant genomes maintain a vast array of chromosomal arrangements ranging from miniature inverted repeat tandem elements and short interspersed regions; to larger genome rearrangements containing inverted repeats. Such regions may be randomly identified by utilizing single primer PCR, also called palindrome or PAL-PCR. In this study, single SSR primers are used in the same manner as RAPD-PCR with the exceptions that: (1) the primers are longer than RAPD oligos; (2) annealing temperatures are higher; and, (3) only amplification products exhibiting one or a few bands are utilized. The amplification products and banding patterns generated are unique to individuals, and of value for identity testing or genotyping studies. Previous application of this approach successfully identified several primers, when used at high stringency conditions, that were associated to potential inverted or quasi-inverted repeat regions in the Poa genome. The approach discovers and exploits the occurrence of PAL's that flank genomic regions of approximately150 bp to 3.0 kb to generate specific and informative genotypes for Poa and perhaps other polyploid grasses. A set of well characterized PAL markers were utilized across six distinct Poa species to investigate their value in determining genetic relatedness.