Submitted to: Annual Review of Analytical Chemistry
Publication Type: Review Article
Publication Acceptance Date: January 19, 2011
Publication Date: June 15, 2011
Citation: Gehring, A.G., Tu, S. 2011. High-throughput biosensors for multiplexed foodborne pathogen detection. Annual Review of Analytical Chemistry. DOI: 10.1146/annurev-anchem-061010-114010. Technical Abstract: Incidental contamination of foods by harmful bacteria (such as E. coli and Salmonella) and the toxins that they produce is a serious threat to public health and the economy in the United States. The presence of such bacteri and toxins in foods must be rapidly determined at various stages of food production, processing and distribution. Producers, processors, regulators, retailers, and public health professionals need simple and cost-effective methods to detect different species of bacteria and their toxins in large numbers of food samples. This review addresses the desire to replace traditional microbiological plate culture with more timely and less cumbersome rapid, biosensor-based methods. Emphasis focuses on high-throughput, multiplex-capable techniques that allow for simultaneous testing of numerous samples, in rapid succession, for multiple foodborne analytes (primarily harmful bacteria and/or toxins).