Submitted to: Omics Technologies and Microbial Modeling in Food-borne Pathogens
Publication Type: Book / Chapter
Publication Acceptance Date: September 28, 2010
Publication Date: January 10, 2012
Citation: Liu, G. 2012. Next generation sequencers: methods and applications in food-borne pathogens. Omics Technologies and Microbial Modeling in Food-borne Pathogens. p. 531-551.
Next generation sequencers are able to produce millions of short sequence reads in a high-throughput, low-cost way. The emergence of these technologies has not only facilitated genome sequencing but also started to change the landscape of life sciences. This chapter will survey their methods and applications in whole-genome sequencing and resequencing, RNA-seq and ChIP-seq ranging from structural, functional and comparative genomics to metagenomics and epigenetics. We begin to witness broad impacts of these next generation sequencers on food safety, including the detection, identification and analysis of food-borne pathogens.