Technical Abstract: Trans-2-hexenal, a volatile aldehyde, is produced by soybean [Glycine max (L.) Merr] and other plants via the lipoxygenase pathway. In vitro tests showed it significantly (p< 0.001) reduced Aspergillus flavus germinating conidial viability at 10 µM, with approximately 95% viability reduction observed at 20µM. The viability of nongerminated conidia were not reduced. Separate experiments were performed to determine the ability of both volatiles to prevent A. flavus growth on sterile corn (20% moisture). Intermittently (15 min. pumping period every 2 hrs) over 5 days both volatiles were pumped through 350 grams of corn that was inoculated with 1 ml of 3 X 10**4 conidia of A. flavus. Controls consisted of sterile corn, corn inoculated with A. flavus with intermittent pumped air, and corn inoculated with A. flavus with no pumped air. Experiments were performed three times for each volatile. Aflatoxin, viability counts and aldehyde concentration in the headspace were performed. A second series of experiments were performed. They were similar to the first series except that trans-2-hexenal (only) was pumped for a 15 min period every 12 hrs. Both experiments showed that intermittent pumping of volatile trans-2-hexenal significantly (p<0.001) prevented A. flavus growth and aflatoxin production over a 7-day period.