ARS | Publication request: Transcript Expression Analysis in Tracheobronchial Lymph Nodes of Pseudorabies Virus Infected Pigs

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: September 27, 2010
Publication Date: December 5, 2010
Citation: Miller, L.C., Harhay, G.P., Bayles, D.O., Zanella, E.L., Lager, K.M. 2010. Transcript expression analysis in tracheobronchial lymph nodes of pseudorabies virus infected pigs [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 115P.

Technical Abstract: This study addresses the critical relationship between Pseudorabies virus (PRV) and its host at a transcriptional level during the course of an infection. RNA isolated from draining tracheobronchial lymph nodes (TBLN) specimens from 5-week old pigs clinically infected with a feral isolate of PRV (FS268) and uninfected were interrogated using Illumina Digital Gene Expression (DGE) Tag Profiling to better understand the physiopathology of infection and the immune response at a cellular level. Over 100 million digital gene expression tag sequences were observed, representing 4,064,189 unique 20-base sequences collected from TBLN at time points 1, 3, 6 and 14 days post-infection. Multidimensional statistical tests were applied to determine which changes in tag abundance were significant and tags were annotated with transcriptomic information. The experimental results have been integrated with previous studies to develop a robust model of swine respiratory virus infection.