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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPING BIOCONVERSION PROCESSES FOR HIGH-VALUE CARBOHYDRATE PRODUCTS

Location: Renewable Product Technology Research Unit

Title: Production of Pullulan, Poly(beta-L-malic acid), and Heavy Oil by Fungus Aureobasidium pullulans Isolated from Thailand

Authors
item Tuwichein, Chalisa -
item Krisraksa, Porntip -
item Eardklay, Sasithorn -
item Leathers, Timothy
item Skory, Christopher
item Pongpoungphet, Napapan -
item Manitchotpisit, Pennapa -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 28, 2010
Publication Date: October 28, 2010
Citation: Tuwichein, C., Krisraksa, P., Eardklay, S., Leathers, T.D., Skory, C.D., Pongpoungphet, N., Manitchotpisit, P. 2010. Production of pullulan, poly(beta-L-malic acid), and heavy oil by fungus Aureobasidium pullulans isolated from Thailand [abstract]. Congress on Science and Technology of Thailand. Abstract #B4 B00090. p. 84.

Technical Abstract: Fungus Aureobasidium pullulans is the main source of a polysaccharide, pullulan, in industrial production. Moreover, it can produce many bioproducts, e.g. xylanase, poly(ß-L-malic acid) (PMA), and heavy oil. In this study, we isolated 15 A. pullulans isolates from various sources and habitats in Thailand and classified using morphological characteristic and sequencing analysis of 2 loci, i.e. ITS (internal transcribed spacer) and BT (beta tubulin). Comparison to database in GenBank, these isolates could be divided into 5 clades indicating the diversity of this fungus in tropical zone. Furthermore, representatives of each clade were selected to study pullulan, PMA, and heavy oil production compared to reference strains. The results showed that A. pullulans from each strain can produce both different yields and characters. This study would be useful for strain selection and development of production from this fungus in the future.

Last Modified: 9/10/2014
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