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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOPMENT OF IMAGING TECHNOLOGY FOR FOOD SAFETY AND SECURITY

Location: Quality and Safety Assessment Research Unit

Title: Detection of pathogens and toxins by surface enhanced Raman spectroscopy using silver nanorods array substrates

Authors
item Huang, Yao-Wen -
item Zhao, Yiping -
item Park, Bosoon

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: December 5, 2009
Publication Date: March 21, 2010
Citation: Huang, Y., Zhao, Y., Park, B. 2010. Detection of pathogens and toxins by surface enhanced Raman spectroscopy using silver nanorods array substrates. American Chemical Society National Meeting.

Technical Abstract: Surface enhanced Raman spectroscopy (SERS) offers considerable potential in the area of molecular identification and trace element analysis, which has significant applications in both biological and chemical analysis. We have created a portable SERS probe which can be easily incorporated with a fiber Raman system. We demonstrated the concentration dependency of Raman intensities on the oblique angle deposition (OAD) fabricated silver nanorod array using BPE as a molecular probe and observed a sensitivity of 14 attomole for BPE. The nanorod substrates were optimized by varying the length, the density and tilting angle of the Ag nanorod arrays by changing the fabrication conditions. The deposition angle will change from 45o to 87o. We have developed a SERS probe that integrated into a fiber Raman system for in situ measurements and act as a portable and remote sensor for accurate and rapid real-time SERS measurements. To test the substrates, we used Escherichia coli and Salmonella typhimurium. The substrate that gives the highest sensitivity and signal-to-noise ratio was optimized.

Last Modified: 4/19/2014
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