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United States Department of Agriculture

Agricultural Research Service

Research Project: CONTROL OF RUSTS OF CEREAL CROPS Title: Cloning and characterization of a wheat ß-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Authors
item Liu, B -
item Xue, X -
item Cui, S -
item Zhang, X -
item Han, Q -
item Zhu, L -
item Liang, X -
item Wang, X -
item Huang, L -
item Chen, Xianming
item Kang, Z -

Submitted to: Molecular Biology Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: September 3, 2009
Publication Date: December 28, 2009
Repository URL: http://www.springerlink.com/content/3624nn5314231731/fulltext.pdf
Citation: Liu, B., Xue, X.D., Cui, S.P., Zhang, X.Y., Han, Q.M., Zhu, L., Liang, X.F., Wang, X.J., Huang, L.L., Chen, X., Kang, Z.S. 2009. Cloning and characterization of a wheat ß-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici. Mol. Bio. Rep. 37:1045-1052.

Interpretive Summary: ß-1,3-glucanases are a group of pathogenesis related protein that have been reported to be involved in plant defense against pathogens in many other plant-pathogen systems. However, it was not clear if these genes play a similar role in wheat against the stripe rust pathogen. To investigate the role of ß-1,3-glucanase in the resistance response of wheat to stripe rust, a wheat ß-1,3-glucanase gene induced by the stripe rust pathogen, designated as TaGlu, was cloned and characterized. The gene was predicted to encode a protein of 334 amino acids. Our results showed that the expression of the gene was induced during both resistant and susceptible responses to the pathogen, but the expression level was much higher in the resistant response than that in the susceptible response. The gene also showed noticeable induction of gene expression in young green leaf tissues treated with salicylic acid, methyl jasmonate or ethepon. The enzyme product of the gene was localized mainly in the plant cell wall and around haustoria, the special structure of the fungus for obtaining water and nutritions from plant cells.The enzyme concentration was found significantly higher in the resistant response than that in the susceptible response. The results show that the cloned ß-1,3-glucanase gene is involved in wheat resistance against stripe rust.

Technical Abstract: Beta-1,3-Glucanases are a group of pathogenesis related proteins that have been reported to be involved in plant defense against pathogens in many other plant pathogen systems. However, it was not clear if these genes play similar role in wheat (Triticum aestivum L.) against Puccinia striiformis f. sp. tritici (Pst), the stripe rust pathogen. To investigate the role of beta-1,3-glucanase (EC 3.2.1.39) in the resistance response of wheat (cv. Suwon 11) to stripe rust, a wheat beta-1,3-glucanase gene induced by Pst, designated as TaGlu, was cloned and characterized. TaGlu was predicted to encode a basic protein of 334 amino acids. Quantitative real-time PCR analyses revealed that the transcription of TaGlu was induced during both compatible and incompatible interactions with Pst, but the transcription level was much higher in the incompatible interaction than that in the compatible interaction. TaGlu also showed noticeable induction of gene expression in young green leaf tissues treated with salicylic acid, methyl jasmonate or ethylene. Immunogold labeling assays showed that the enzyme were localized mainly in the host cell wall and over the extra haustorial matrix, and the labeling densities were found significantly higher in the incompatible interaction than those in the compatible interaction.

Last Modified: 10/25/2014
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