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United States Department of Agriculture

Agricultural Research Service

Research Project: ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES

Location: Bioenergy Research Unit

Title: Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification

Authors
item Barr, Christopher -
item Wissinger, Joshua -
item Samayam, Indira -
item Schall, Constance -
item Mertens, Jeffrey

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: November 13, 2010
Publication Date: November 13, 2010
Citation: Barr, C.J., Wissinger, J., Samayam, I.P., Schall, C.A., Mertens, J.A. 2010. Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification [abstract]. American Institute of Chemical Engineers. p. 265.

Technical Abstract: Biomass, which consists of cellulose, hemicellulose, and lignin, is being developed as a renewable carbon source for fuel and chemical production. The use of biomass as a renewable carbon source requires that it be hydrolyzed to monomeric sugars through efficient processes. The process which shows the most promise for the deconstruction of biomass is pretreatment via chemical processes followed by enzymatic hydrolysis. Enzymatic hydrolysis is preferred over mineral acid catalyzed hydrolysis because no sugar breakdown products, which often times act as inhibitors in downstream processes, are produced. The continued optimization of the biofuel production from terrestrial biomass includes development of processes that takes advantage of fundamental understanding of the complex interplay between lignocellulose components. Ionic liquid (IL) pretreated biomass provides an opportunity to improve hydrolysis processes through increased understanding of the interactions between lignocellulosic components since it is a unique feedstock in that it provides amorphous cellulose while retaining the hemicellulose and lignin components. In addition, enzyme groups (cellulases and hemicellulases) which are critical for saccharification of this substrate are identified. Some of these enzyme groups are endoglucanases, cellobiohydrolases, beta-glucosidases, xylanases, and xylosidases. Selected cellulases and hemicellulases are expressed, isolated, purified, and characterized for activity and substrate specificity. The identified enzyme systems are also examined for their synergistic effects on the hydrolysis of ionic liquid pretreated biomass.

Last Modified: 9/22/2014
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