Technical Abstract: After removal of soluble sugars and other compounds by washing, citrus peel is largely composed of pectin, cellulose and hemicellulose. One of the major components, pectin can be modified using pectinesterases which reduces the degree of methylation (DM) to produce lower DM pectins which have great utility in the food industry and other applications. These lower DM pectins have been shown to have calcium sensitivity which is an important functional property of pectins for use in applications which require suspension, metal ion binding or water absorption. Thus the measurement of degree of methylation is an important attribute. Reduction in DM results in production of carboxyl groups which are ionic and conductive. Data shows that the conductivity as measured by CC4 conductivity meter increases with decreasing DM with values ranging from 94.0 to zero when pectins are analyzed on size exclusion chromatography columns (SEC) with concentration data measured with interferometric refractive index detector (RI). Best data fit is linear equation of y = -5.96 x 10-3 + 0.592 correlation coefficient of 0.998. Data demonstrates the DM can be measured simultaneously with molecular weight on SEC.