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United States Department of Agriculture

Agricultural Research Service

Research Project: FUNCTIONAL GENOMICS FOR IMPROVING NUTRIENTS AND QUALITY IN ALFALFA AND SOYBEAN

Location: Plant Science Research

Title: Fast Neutron Mutagenesis of Soybean: A Resource for the Community

Authors
item Bolon, Yung Tsi
item Haun, William -
item Muehlbauer, Gary -
item Orf, James -
item Naeve, Seth -
item Stupar, Robert -
item Vance, Carroll

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 10, 2010
Publication Date: August 8, 2010
Citation: Bolon, Y.E., Haun, W., Muehlbauer, G., Orf, J., Naeve, S., Stupar, R., Vance, C.P. 2010. Fast Neutron Mutagenesis of Soybean: A Resource for the Community [abstract]. 13th Biennial Molecular and Cellular Biology of the Soybean Conference, August 8-11, 2010, Durham, North Carolina.

Technical Abstract: Mutagenized populations have become indispensable resources for introducing variation and studying gene function in plant genomics research. To create a soybean population for forward and reverse genetic screens, we chose to use fast neutron irradiation to induce deletion mutations in the soybean genome. Here, we describe the development of a fast neutron mutagenized soybean population. We exposed approximately 120,000 soybean seed to fast neutron irradiation doses of up to 32 Gy and developed more than 20,000 unique M2 lines. Moreover, we demonstrate the utility of this population for phenotypic screening and associated genomic characterization of striking and agronomically important traits. We catalogued plant variation in seed composition, maturity, morphology, pigmentation, roots, and nodulation. Notably, we identified mutants that show significant increases or decreases in seed protein and oil content across three generations and environments. Finally, we applied comparative genome hybridizations to reveal deletion regions and candidate genes associated with selected phenotypes of interest. We present this fast neutron mutant soybean population as a valuable resource for future forward and reverse genetic screens.

Last Modified: 9/23/2014
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