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United States Department of Agriculture

Agricultural Research Service

Research Project: Improving Biochemical Processes for the Production of Sustainable Fuels and Chemicals

Location: Renewable Product Technology Research Unit

Title: Engineered Cellulosic Yeast for Direct Production of Energy-Dense, Infrastructure-Compatible Fuels from CO2 and Cellulosic Sugars


Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: June 3, 2010
Publication Date: June 3, 2010
Citation: Hughes, S.R. 2010. Engineered cellulosic yeast for direct production of energy-dense, infrastructure-compatible fuels from CO2 and cellulosic sugars [abstract]. BioConference Live.

Technical Abstract: Strains of yeast expressing novel lipase will be engineered to produce two energy-dense liquid fuels using an improved column-based process for production of biodiesel and alcohol was developed using a column containing a strongly basic anion-exchange resin in sequence with a column containing a resin to which a lipase biocatalyst is bound. Currently most biodiesel is produced by transesterification of triglycerides, such as corn oil, with short-chain alcohols, such as methanol or ethanol, with a strong base as a catalyst. The transesterification reaction to produce biodiesel can also be accomplished using resin-bound lipases derived from yeast systems and used as biocatalysts for biodiesel production from row crops or from algae.

Last Modified: 8/27/2014
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