Evaluations of Fusarium wilt resistance in Upland cotton from Uzbek cotton germplasm resources.

Authors: ABDULAEV, AALISHER - Uzbekistan Institute Of Genetics; SALAHUTDINOV, ILHOM - Uzbekistan Institute Of Genetics; KURYAZOV, ZARIF - Uzbekistan Institute Of Genetics; EGAMBERDIEV, SHAROF - Uzbekistan Institute Of Genetics; RIZAEVA, SOFIA - Uzbekistan Institute Of Genetics; ADYLOVA, AZODA - Uzbekistan Institute Of Genetics; ABDUKARIMOV, ABDUSATTOR - Uzbekistan Institute Of Genetics; Ulloa, Mauricio; ABDURAKHMONOV, IBROKHIM - Uzbekistan Institute Of Genetics

Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/2010
Publication Date: 9/21/2010
Citation: Abdulaev, A., Salahutdinov, I., Kuryazov, Z., Egamberdiev, S., Rizaeva, S., Adylova, A.T., Abdukarimov, A., Ulloa, M., Abdurakhmonov, I.Y. 2010. Evaluations of Fusarium wilt resistance in Upland cotton from Uzbek cotton germplasm resources. International Cotton Genome Initiative Workshop. Page 64.

Interpretive Summary:

Technical Abstract: Fusarium oxysporum f. sp. vasinfectum Atk. Sny & Hans (FOV), in combination with Verticillium dahliae Kleb, causes a wilt disease complex in cotton that significantly reduces yield. A highly virulent strain of FOV, No. 316, was isolated that caused up to 80% plant death in commercial cotton in Uzbekistan. We developed an experimental F2:3 cotton population by crossing a Mexican cultivar, Mebane-1B, (wilt resistant) and the germplasm line No.11970 (susceptible) and assessed response to FOV in two studies. One study used an artificial inoculation of strain FOV No. 316 in a field known to be infested by FOV, and the second study used an artificial inoculation of FOV No. 316 in an uninfested field. In the evaluation including natural FOV infestation the observed phenotypic segregation pattern was about 3:1 (susceptible:resistant) whereas in the second study the segregation ratio was about 1:1. The observed segregation ratios in the two studies suggested a difference in response to natural and artificial infestations of FOV. These results are consistent with previous reports. We confirmed the presence of FOV isolate No. 316 from infected plants in the naturally infested field, but we also isolated a strain of Verticillium from the same field. Therefore, observed segregation ratios should be interpreted with caution. Molecular screening efforts with 526 SSR primer-pairs yielded 69 polymorphic SSRs between susceptible germplasm line No. 11970 and resistant cultivar Mebane-1. Phenotypic and marker data are being used to further investigate inheritance of resistance, the number of involved genes, and to construct associated genetic and QTL maps.