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United States Department of Agriculture

Agricultural Research Service

Research Project: EFFICIENT MANAGEMENT AND USE OF ANIMAL MANURE TO PROTECT HUMAN HEALTH AND ENVIRONMENTAL QUALITY

Location: Food Animal Environmental Systems Research Unit

Title: Correlating attachment behavior with cell properties for eight Porcine Escherichia coli Isolates

Authors
item Bolster, Carl
item Cook, Kimberly
item Marcus, Ian -
item Haznedaroglu, Berat -
item Walker, Sharon -

Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 28, 2010
Publication Date: November 1, 2010
Citation: Bolster, C.H., Cook, K.L., Marcus, I.M., Haznedaroglu, B.Z., Walker, S.L. 2010. Correlating attachment behavior with cell properties for eight Porcine Escherichia coli Isolates. ASA-CSSA-SSSA Annual Meeting Abstracts.

Technical Abstract: In this study we investigate how growth stage and depositional environment affect variability of cell properties and transport behavior of eight porcine E. coli isolates. We compared the surface properties and transport behavior for cells harvested at two different growth stages (exponential and stationary) and depositional environments (favorable and unfavorable). We then investigated correlations between measured cell properties and fitted bacterial attachment efficiencies. For both growth stages we found that bacterial attachment efficiencies to negatively-charged quartz sand varied among the eight different isolates by over an order of magnitude whereas attachment efficiencies to the positively-charged Fe-coated sands varied by a factor of less than two. With the exception of one isolate, growth condition had minimal impact on attachment efficiencies to the uncoated sands. A strong and statistically significant inverse relationship was observed between bacterial attachment to uncoated quartz sand and log-transformed zeta potential whereas a mild yet statistically significant relationship between bacterial attachment to the Fe-coated soils and cell width was observed. For the experimental conditions used in our study, we found that variability in E. coli transport was more dependent on the depositional environment than on growth conditions.

Last Modified: 4/18/2014