ARS | Publication request: Screening for Recombinant Avian Leukosis Viruses in Cell Cultures Inoculated with Various Subgroups of Virus

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: August 1, 2010
Publication Date: August 1, 2010
Citation: Fadly, A.M., Mays, J.K., Hunt, H.D. 2010. Screening for Recombinant Avian Leukosis Viruses in Cell Cultures Inoculated with Various Subgroups of Virus [abstract]. In: American Association of Avian Pathologists Symposium and Scientific Program, August 1-4, 2010, Atlanta, Georgia. Paper No. 9271.

Technical Abstract: Chicken embryo fibroblasts (CEFs) prepared from ADOL SPF embryos were co-infected with different concentration ratios of subgroups A, J and E avian leukosis virus (ALV). Inoculated cultures were screened for recombination among the ALV strains. Potential recombinant viruses were purified by limiting dilution and then screened for subgroup on virus restrictive cell lines. Data suggest that recombination among tested subgroups can occur, and that purification by limited dilution and use of restrictive cell lines are essential for screening potential recombinant ALVs. The use of envelope and LTR specific PCR primer sets in screening potential recombinant ALVs will also be discussed. .