Title: Ultra high-throughput nucleic acid sequencing as a tool for virus discovery in the turkey gut. Authors
Submitted to: Feedinfo News Service
Publication Type: Trade Journal
Publication Acceptance Date: April 16, 2010
Publication Date: April 19, 2010
Citation: Day, J.M., Zsak, L. 2010. Ultra high-throughput nucleic acid sequencing as a tool for virus discovery in the turkey gut. Available: http:www.feedinfo.com. Technical Abstract: Recently, the use of the next generation of nucleic acid sequencing technology (i.e., 454 pyrosequencing, as developed by Roche/454 Life Sciences) has allowed an in-depth look at the uncultivated microorganisms present in complex environmental samples, including samples with agricultural importance. This new technology allows researchers to gather and analyze a large amount of nucleic acid sequence data for a specific organism (genomics) or to compile sequence information for the multiple organisms present in a more complex sample (metagenomics). We have recently applied high-throughput pyrosequencing to prepare a turkey gut RNA virus metagenome and to discover known and novel RNA viruses in the turkey gut, specifically from turkey farms and flocks that have experienced a historically high incidence of enteric signs and performance problems.