ARS | Publication request: Multi-Locus Analysis of a Citreoviridin-Producing Isolate Previously Identified as Penicillium NRRL 13013

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 6, 2010
Publication Date: August 6, 2010
Citation: Peterson, S.W. 2010. Multi-Locus Analysis of a Citreoviridin-Producing Isolate Previously Identified as Penicillium NRRL 13013. Meeting Abstract.

Technical Abstract: Cole et al (1981) reported a citreoviridin-producing isolate of Penicillium charlesii (NRRL 13013) from molded pecans. Wicklow later identified it as a variant of Penicillium citreoviride, noting that it produced sclerotia, although the species as a whole is not known to do so. We sequenced the ITS region of NRRL 13013 to assess its phylogenetic relationships to Penicillium and conducted a multilocus congruence study of this isolate and several others hypothesized to be related on the basis of the ITS sequence. Loci sequenced were beta-tubulin, calmodulin, RNA polymerase beta, Mcm7 and Tsr1. Congruence analysis of the phylogenetic trees based on each locus revealed the presence of seven previously undescribed species in a clade that also includes Eupenicillium idahoense, P. malacaense, P. parvulum and E. cinnamopurpureum. Another noted citreoviridin-producing species, Eupenicillium ochrosalmoneum is not in the same clade as NRRL 13013. The outstanding feature of the species in this clade of monoverticillate species is the very slow growth rate (5-10 mm diam after 7 days on CYA). Microscopic similarities are great, but colony appearance and colors are quite distinct for each species.