Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 2, 2010
Publication Date: March 16, 2010
Citation: Long, J.A. 2010. Chapter VI. Semen cryopreservation. Section 3. Glycerol removal from thawed semen. In: Bakst, M.R., Long, J.A., editors. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. 2nd edition. St. Paul, MN: The Midwest Poultry Federation. p. 68-71.
Interpretive Summary: This is a protocol for removing glycerol from thawed poultry semen prior to insemination. The advantages of this method versus serial dilution include fewer dilutions and centrifugations and a more complete reduction in glycerol content. However, this protocol does require the use of a refrigerated centrifuge to maintain temperature during the long centrifugation. The following protocol is based on the original publication from McLean et al., (1998).