Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: March 2, 2010
Publication Date: March 16, 2010
Citation: Long, J.A. 2010. Chapter VI. Semen cryopreservation. Section 2. Cryopreservation of rooster sperm using DMA. In: Bakst, M.R., Long, J.A., editors. Techniques for Semen Evaluation, Semen Storage, and Fertility Determination. 2nd edition. St. Paul, MN: The Midwest Poultry Federation. p. 65-67.
Interpretive Summary: This is a general protocol for cryopreservation of rooster sperm using dimethylacetamide (DMA) as the cryoprotectant. One advantage of using a DMA-based diluent is that the cryoprotectant does not have to be removed from thawed semen prior to insemination. Dimethylacetamide is most effective at very fast freezing rates achieved with the pellet method. Use of straw packaging in conjunction with DMA is still being investigated.