ARS | Publication request: Characterization of Folate in Peanuts

Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: April 1, 2010
Publication Date: N/A

Technical Abstract: The folate levels in a group of raw and roasted samples selected from the 2007 and the 2008 Uniform Peanut Performance Trials (UPPT) and from a set of raw samples from the Core of the Core of the Peanut Germplasm collection grown in 2006 and 2008 were determined. The samples were digested in protease and amylase to free the vitamers from the matrix. The homogenized samples were treated with an additional enzyme to deconjugate the polyglutamates. The different monomers of the folates present were determined using High Performance Liquid Chromatography coupled to Mass Spectrometry (HPLC-MS). Raw samples from the UPPT were found to have significantly higher levels of total folates compared to roasted. There were significant differences between years for the Core of the Core samples regardless of the variety. The predominate vitamers found were 5-methyl-tetrahydrofolate and 5-formyl-tetrahydrofolate regardless of origin, variety or year.